Journal: Nature Communications

Article Title: VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination

doi: 10.1038/ncomms13478

Figure Lengend Snippet: ( a ) Tissue lysates from 7-, 21- and 28-day-old CD69 shRNA transgenic (Tg) and control mouse brains were immunoblotted with an antibody against MBP, CNPase, CD69, VCAM1 or actin. Data are representative of three experiments. ( b ) Corpus callosum sections at postnatal day 11 were co-stained with an anti-MBP antibody (red) and DAPI (blue). Data are representative of two experiments. Scale bar, 200 μm. ( c – e ) Electron microscopic data of 9-day-old corpus callosum are shown. Data are representative. Scale bar, 2 μm. The g-ratios (200–300 myelinated fibres) are plotted for axon diameters. Average g-ratios are also shown. Data were evaluated using Student’s t -test (** P =7.13E−15; n =213–260 axons of two independent experiments).

Article Snippet: MBP promoter-driven Cre recombinase transgenic mice (RBRC No. 01461) were obtained from Riken BioResource Center (Ibaraki, Japan).

Techniques: shRNA, Transgenic Assay, Staining